Biosimilar App Notes & Case Studies
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Recombinant Adeno-Associated Virus Type 5 Production Process
10/12/2022
Learn about a scalable process for the production of rAAV using triple plasmid transfection of suspension cells in an animal-derived component-free medium.
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Scalability And Performance Of Single-Use Bioreactors
8/1/2023
Here, we provide scalability data as a guide for process development and cross-scale performance expectations and characterize performance attributes of the impeller, spargers, Ultimus® film, and sensors.
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Alkaline Stability Of Modern Protein A Chromatography Resins
7/12/2023
Explore results from a resin lifetime study with over 150 cycles, using clarified cell harvest in every cycle, which has been performed to evaluate the alkaline stability of three protein A resins.
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Exosome Isolation By TFF And Size Exclusion Chromatography
3/6/2024
Here, we demonstrate a scalable workflow for the isolation of exosomes that combines tangential flow filtration for the concentration of exosomes followed by gentle size exclusion chromatography.
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Generating A Robust Host Cell Line For A Cell Line Development Platform
11/14/2022
Learn how cell performance within the context of the cell line development workflow was confirmed with the successful isolation of stable, high-producing clones from three model monoclonal antibodies.
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Depth Filtration Devices For Cell Cultures, Fermentation Broths, And Cell Lysates
3/27/2023
Typically, devices containing depth media filters can offer more efficient filtration. Browse depth filtration products and devices for the downstream processing of cell culture solutions, fermentation broths, and lysates.
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Higher Order Structure Of Proteins By Circular Dichroism Spectroscopy
9/30/2022
Learn more about the analytical development for mAbs and recombinant proteins
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AAV Full/Empty Capsid Separation Using Mechanistic Modeling
5/1/2023
In this study, we used mechanistic modeling to investigate how AAV-resin interactions are affected when MgCl2 is used as an elution additive.
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Lentiviral Vector Production Using Single-Use Bioreactors
5/6/2024
Here, we outline a scalable and robust upstream process developed for LVV production of HEK293T cells and demonstrate how to expand HEK293T suspension cells.
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Performing A Tangential Flow Filtration (TFF) Optimization Study
2/14/2023
This application note describes the recommended methods and techniques for optimizing a tangential flow filtration (TFF) process using a Cogent® Lab system and the Bio4C® Control Software.