Biosimilar App Notes & Case Studies
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Performing A Tangential Flow Filtration (TFF) Optimization Study
2/14/2023
This application note describes the recommended methods and techniques for optimizing a tangential flow filtration (TFF) process using a Cogent® Lab system and the Bio4C® Control Software.
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Bioreactor Scalability Supports Intensified Upstream Process Development
7/31/2023
In this study, key parameters for the Mobius® 3 L Single-Use Bioreactor and the 50-2000 L Mobius® iFlex Bioreactor impeller designs were characterized. Learn about bioreactor scalability for intensified processes.
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AAV Production In Suspension HEK293 Cells With Single-Use Bioreactors
10/13/2022
Explore how we expanded HEK293/HEK293T suspension cells from small cell culture (20 mL) in shake flasks up to a 10 L culture in a benchtop Xcellerex™ XDR-10 single-use bioreactor system.
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Trends In Next-Generation Delivery Technologies
11/22/2023
Learn how lipid nanoparticles are enabling a new generation of engineered cell therapies with a push toward more complex cell engineering and gene editing for allogeneic therapies.
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Process Optimization And Scalability Evaluation Of PelliconĀ® Capsules For SPTFF Of mAb-Based Biomolecules
6/17/2022
This document describes the principles of SPTFF and how to determine operating conditions to achieve the target concentration factor with single-use Pellicon® Capsules.
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Enhancing Ease Of Use Batch Turnaround And Process Flexibility With Single-Use TFF
6/17/2022
Use of single-use Pellicon® Capsules is an effective strategy for improving operational flexibility, product safety, and turnaround of a TFF step.
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Biacoreā¢ SPR Systems For Titer Analysis Of Adeno-Associated Virus
10/13/2022
We describe the performance of two assays for titer analysis of adeno-associated virus (AAV), serotype 2 and serotype 5, based on surface plasmon resonance (SPR).
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Optimizing The Formulation For A Monoclonal Antibody
8/21/2023
An emerging biotech company sought help developing a formulation for a monoclonal antibody. Explore how MilliporeSigma delivered an optimized formulation within an aggressive timeline and budget.
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TFF For Laboratory And Process Development Applications
3/1/2023
Learn about the basic principles that govern tangential flow filtration (TFF) along with the use of TFF capsules and cassettes in laboratory and process development applications.
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Exosome Isolation By TFF And Size Exclusion Chromatography
3/6/2024
Here, we demonstrate a scalable workflow for the isolation of exosomes that combines tangential flow filtration for the concentration of exosomes followed by gentle size exclusion chromatography.